Saturday, August 7, 2021

BIO warfare (anthrax)

According to the jihadi forum, the following factors make anthrax the weapon of choice:
 
• Anthrax is powerful, lethal, cheap and easy to prepare.
• 50 grams of anthrax, when dispersed in a 2-kilometer line, forms a deadly cloud that can cover 20 kilometers.
• One kilogram of anthrax can be produced in a small laboratory in 96 hours.
• Anthrax bacteria spores are available worldwide and can be easily extracted.
• Production costs are low; one kilogram of anthrax bacteria costs about $50 even though a lethal dose can be as little as one millionth of a gram.
• Colorless and odorless anthrax is easily concealed.
• Anthrax is a stable and dry substance that can be easily transported and used.
 

The protocol used for soil sample processing optimization consisted of adding 200 mL of diluent to a 45 g soil sample inoculated with spores, shaking for 3 min, and settling for 15–30 min. The supernatant was then transferred to a 250 mL Sorvall® centrifuge bottle (Cat #03937) and centrifuged at 250 × g for 5 min to remove large particles. The supernatant was transferred to a new bottle and the sample was centrifuged at 5900 × g for 35 min to pellet the spores. The pellet was resuspended in 25 mL diluent and transferred to a 50 mL centrifuge tube and centrifuged at 5900 × g for 35 min to pellet the spores. The pellet was resuspended in 2.2 mL diluent and heat treated for one hour at 70 °C. 150 μL was used for plating


INTEL : 



 bom dia!


embora as secretas procurem quem produz em laboratório ou homemade anthrax, é um facto, que é mais "fácil" encontrar no ambiente.

se as cabras do monte, na regiao do Afeganistao, sao as grandes "produtoras" da bactéria...

outras partes do mundo, (link abaixo ) tem com facilidade , a oportunidade de recolher amostras.

sendo, uma bactéria que se desnevolve pelas fezes e urinas de animais, sobretudo animais selvagens.

será que no zoo de Lisboa, encontramos anthrax?

com certeza que sim

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