According to the jihadi forum, the following factors make anthrax the weapon of choice:
• Anthrax is powerful, lethal, cheap and easy to prepare.
• 50 grams of anthrax, when dispersed in a 2-kilometer line, forms a deadly cloud that can cover 20 kilometers.
• One kilogram of anthrax can be produced in a small laboratory in 96 hours.
• Anthrax bacteria spores are available worldwide and can be easily extracted.
• Production costs are low; one kilogram of anthrax bacteria costs about $50 even though a lethal dose can be as little as one millionth of a gram.
• Colorless and odorless anthrax is easily concealed.
• Anthrax is a stable and dry substance that can be easily transported and used.
The protocol used for soil sample processing optimization consisted of adding 200 mL of diluent to a 45 g soil sample inoculated with spores, shaking for 3 min, and settling for 15–30 min. The supernatant was then transferred to a 250 mL Sorvall® centrifuge bottle (Cat #03937) and centrifuged at 250 × g for 5 min to remove large particles. The supernatant was transferred to a new bottle and the sample was centrifuged at 5900 × g for 35 min to pellet the spores. The pellet was resuspended in 25 mL diluent and transferred to a 50 mL centrifuge tube and centrifuged at 5900 × g for 35 min to pellet the spores. The pellet was resuspended in 2.2 mL diluent and heat treated for one hour at 70 °C. 150 μL was used for plating
INTEL :
Last Updated. 03 Nov 2002: Source: Baltimore Sun, November 3, 2002. Anthrax powder from attacks could have been made simply. Single maker a possibility, scientists now theorize www.ph.ucla.edu |
bom dia!
Introduction. Anthrax outbreaks occur world-wide and are of concern for animal conservation (Leendertz et al. 2004, Muoria et al. 2007), wildlife management (Clegg et al. 2007, Wafula et al. 2008), agriculture (Mongoh et al. 2008, Durrheim et al. 2009, Lewerin et al. 2010) and public health (Siamudaala et al. 2006).Anthrax is caused by the bacterium Bacillus anthracis which can infect a wide ... esajournals.onlinelibrary.wiley.com |
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