Sunday, May 14, 2017

MUSE - Aftermath [Official Music Video]

HUMANKIND IMMORTALITY INSIDE A GAS BUBBLE

The aim of the present study was to examine the feasibility of DNA microarray technology in an attempt to construct an evaluation system for determining gas toxicity using high-pressure conditions, as it is well known that pressure increases the concentration of a gas. As a first step, we used yeast (Saccharomyces cerevisiae) as the indicator organism and analyzed the mRNA expression profiles after exposure of yeast cells to nitrogen gas. Nitrogen gas was selected as a negative control since this gas has low toxicity. Yeast DNA microarray analysis revealed induction of genes whose products were localized to the membranes, and of genes that are involved in or contribute to energy production. Furthermore, we found that nitrogen gas significantly affected the transport system in the cells. Interestingly, nitrogen gas also resulted in induction of cold-shock responsive genes. These results suggest the possibility of applying yeast DNA microarray to gas bioassays up to 40 MPa. We therefore think that "bioassays" are ideal for use in environmental control and protection studies.
Key words: DNA microarray, Yeast, Hydrostatic pressure, Gas pressure, Nitrogen gas

Introduction 
While seeking a comfortable living environment, the human race has been continuously releasing large quantities of pollutants into the environment, consequently causing various problems such as air, water, and soil pollution. Although emission of pollutants is being gradually reduced by the recent tighter control of emissions of environmental pollutants, we are still polluting our environment. The problem is further compounded by the fact that we are not dealing with a single pollutant, but with a mixture of pollutants in the environment, and therefore it is rather difficult to recognize their effects on organisms. Methods that can determine their effect primarily include physiochemical analyses and bioassays. When pollution is supposed to be present to some extent, we can use physicochemical analyses as a qualitative and quantitative tool for a single substance; however, this is not useful for the assessment of the impact of tens of millions of chemical substances on the environment. Although we cannot determine the concentration of each chemical substance by bioassay, it is possible to do rapid toxicity and genetic toxicity analysis using animal organisms (mammals such as rodents, fish and shellfish, algae, single cells, etc.) because it focuses attention on the effect on organisms.
We have studied a bioassay for the hydrosphere using a yeast DNA microarray. In the present study, drawing on our previous experience, we attempted to develop a bioassay system for polluted air monitoring. By using yeast (Saccharomyces cerevisiae S288C), suitable for rapid toxicity assessment, we can monitor genome-wide gene expression profiles by DNA microarray after exposing yeast cells to high-pressure gas. Thus, we believe it will be possible to assess the impact of polluted gases within short periods of time.
In the present report, we focus mainly on nitrogen gas, a component of our life-supporting air in the environment. Our results revealed significant changes in gene expression profiles, such as those involved in the membrane system, energy, cellular transport, and even cold-response. Since we were able to detect and characterize the response, we conclude that we may use high-pressure gas conditions for gas bioassay up to 40 MPa.


Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
US 7033781 B1
RESUMO
An invention comprising cellular transformation, directed evolution, and screening methods for creating novel transgenic organisms having desirable properties. Thus in one aspect, this invention relates to a method of generating a transgenic organism, such as a microbe or a plant, having a plurality of traits that are differentially activatable. Also, a method of retooling genes and gene pathways by the introduction of regulatory sequences, such as promoters, that are operable in an intended host, thus conferring operability to a novel gene pathway when it is introduced into an intended host. For example a novel man-made gene pathway, generated based on microbially-derived progenitor templates, that is operable in a plant cell. Furthermore, a method of generating novel host organisms having increased expression of desirable traits, recombinant genes, and gene products.


talking about Jesus miracles again...check it out:

talking about Jesus miracles again...check it out:
The first photosynthetic organisms probably evolved early in the evolutionary history of life and most likely used reducing agents such as hydrogen or hydrogen sulfide, rather than water, as sources of electrons.[6] Cyanobacteria appeared later; the excess oxygen they produced contributed directly to the oxygenation of the Earth,[7] which rendered the evolution of complex life possible. Today, the average rate of energy capture by photosynthesis globally is approximately 130 terawatts,[8][9][10] which is about three times the current power consumption of human civilization.[11] Photosynthetic organisms also convert around 100–115 thousand million metric tonnes of carbon into biomass per year...


Elsa David So, Jesus had a particulary use of this connections in science protein DLD H. sapiens Energy metabolism Protease


here's the mystery
Fragmentation or clonal fragmentation in multi cellular or colonial organisms is a form of asexual reproduction or cloning in which an organism is split into fragments. Each of these fragments develop into mature, fully grown individuals that are clones of the original organism.
The splitting may or may not be intentional – it may or may not occur due to man-made or natural damage by the environment or predators
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Elsa David first question how did Jesus used light energy for clonal fragmentation, second question how to do this in less than 30 seconds
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Elsa David Binary fission, asexual reproduction by a separation of the body into two new bodies. In the process of binary fission, an organism duplicates its genetic material, or deoxyribonucleic acid (DNA), and then divides into two parts (cytokinesis), with each new organism receiving one copy of DNA.

the binary fission was made by Jesus with some sort of magnetic technique


Elsa David question is, did he had a magnetic force on his biology that other humans can have and not know
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Elsa David do fragmentation happens by magnetism on our hands trought light energy passing trought
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Elsa David I'll be back later
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Elsa David just another question does magnesium on our biology composition makes the difference between the ability of duplication of materia?
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Elsa David one of these days, with some money for pizza dinners, I'll write a novel "The Last Man Thinking"
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Elsa David on the Lab for this duplication fragmentation binary fission of human DNA totally grow takes one month
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Elsa David Humankind is 99.9% stupid! people simple disbelievle Jesus just because they do, and some believe just because they do
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Elsa David who am I ...a nobody
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Elsa David Descartes "I think, therefore I exist"
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Elsa David the beggining of the universe, like on the Bible "In the beggining there was nothing" yes there was vaccum of gas...and the gas simple created by force of electrons chocking, a virus,,, and we are the product of the methamorfose of that virus
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Elsa David that virus, that bacteria mutation, is called on the last argument, humans
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Elsa David the bacteria imploded on the super nova
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Man in the Rain