welcome back to war Jihad! You have at least 6 guys on your secret lab, however the cartles and east mafia hv much more. Let's go for cholera preparation:
Thiosulphate Citrate Bile Salts Agar (TCBS)
(Oxoid designation is Cholera Medium TCBS)
Basic ingredients
Peptone 10,0 g
Yeast extract 5,0 g
Sodium citrate 10,0 g
Sodium thiosulfate.5 H2O (Na2S2O3•5 H2O) 10,0 g
Sodium chloride (NaCl) 10,0 g
Ox gall powder 5,0 g
Iron (III) citrate (FeC6H5O7) 1,0 g
Sodium cholate 3,0 g
Sucrose 20,0 g
Thymol Blue solution (0,2%) 20,0 ml
Bromothymol Blue solution (0,2%) 20,0 ml
Agar 15,0 g
Distilled water 1,0 L
pH 8,6 ± 0,2
Preparation
Mix all ingredients and apply heat until all goes into solution. DO NOT STERILIZE USING AUTOCLAVE. Allow to cool to 45-50°C and distribute.
Notes:
from http://www.disknet.com/indiana_biolab/b041.htm:
Peptones are the most widely used source of nitrogen in microbial media. Some are made by cooking milk or meat products in acid, but most are made by incubating milk or meat with trypsin, pepsin, or other proteolytic enzymes to digest the protein to a mixture of amino acids, peptides, and polypeptides. Many microbes, called proteolytic, can digest proteins, but most can't. The choice of peptone is sometimes of importance.
Some water-based inks contain ox gall (bile) as a dispersant. Pure ox gall can be obtained from art supply stores (eg. Winsor & Newton brand).
Sodium cholate, a bile salt, is used as an anionic detergent
http://www.sciencemadness.org/talk/viewthread.php?tid=1485&page=2
Thiosulphate Citrate Bile Salts Agar (TCBS)
(Oxoid designation is Cholera Medium TCBS)
Basic ingredients
Peptone 10,0 g
Yeast extract 5,0 g
Sodium citrate 10,0 g
Sodium thiosulfate.5 H2O (Na2S2O3•5 H2O) 10,0 g
Sodium chloride (NaCl) 10,0 g
Ox gall powder 5,0 g
Iron (III) citrate (FeC6H5O7) 1,0 g
Sodium cholate 3,0 g
Sucrose 20,0 g
Thymol Blue solution (0,2%) 20,0 ml
Bromothymol Blue solution (0,2%) 20,0 ml
Agar 15,0 g
Distilled water 1,0 L
pH 8,6 ± 0,2
Preparation
Mix all ingredients and apply heat until all goes into solution. DO NOT STERILIZE USING AUTOCLAVE. Allow to cool to 45-50°C and distribute.
Notes:
from http://www.disknet.com/indiana_biolab/b041.htm:
Peptones are the most widely used source of nitrogen in microbial media. Some are made by cooking milk or meat products in acid, but most are made by incubating milk or meat with trypsin, pepsin, or other proteolytic enzymes to digest the protein to a mixture of amino acids, peptides, and polypeptides. Many microbes, called proteolytic, can digest proteins, but most can't. The choice of peptone is sometimes of importance.
Some water-based inks contain ox gall (bile) as a dispersant. Pure ox gall can be obtained from art supply stores (eg. Winsor & Newton brand).
Sodium cholate, a bile salt, is used as an anionic detergent
http://www.sciencemadness.org/talk/viewthread.php?tid=1485&page=2
